Bmon14272序列
Web(bMON14272) were prepared as described by Motohashi et al. (2005) and were then transfected with BT3Bac by electroporation. A kanamycin-resistant and lacZ-positive colony was selected and designated BmT3DH10Bac (Fig.1). Rescue of polyhedrin gene (polh) in BmNPV/T3 bacmid (BT3Bac) To generate a polh-rescued BT3Bac, a polyhedrin gene Webcontain the parent bacmid bMON14272 and the helper plasmid pMON7124. See the Elements table on page 4 for composition details. A composite bacmid molecule is …
Bmon14272序列
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WebCapable of supporting site-specific recombination between pFastBac™ and bMON14272. Supplier: Invitrogen™ 10361012. Catalog No. 10-361-012. $658.00 / Each of 1. Qty Check Availability. Add to cart. Includes: MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100μL each (total of 500μL), pUC19 DNA (0.01 μg/mL): 1 vial, 100μL, SOC ... WebDownload scientific diagram Detection and isolation of the composite bMON14272::pFASTBac1/ α 7. a) DH10Bac colonies grown in selective medium supplemented with X-Gal, after transformation with ...
http://wolfson.huji.ac.il/expression/bac.pdf Webbmon14272 from the remaining culture and store at –80°C. D. Prepare Lambda-Red Electrocompetent Cells 1. Chill 20 mL deionized water in a 50 mL conical tube on ice. 2. Add 10 mL of LB media with 10 µL of 50 mg/mL kanamycin to a 50 mL baffled Erlenmyer flask. 3. Inoculate the flask with 250 µL of the remaining SW106-bmon14272 culture. 4.
WebSep 1, 2010 · (A) PCR products, generated from template bMON14272 and primer pairs 6054–6055 and 6059–6060, containing 3′ and 5′ gp64 flanking sequences were fused in an Overlap-PCR with primer pair ... WebNov 10, 2013 · 杆状病毒表达系统helper质粒与Bacmid质粒序列及图谱. 如题，求助杆状病毒表达系统helper质粒（pMON7124）与Bacmid质粒（bMON14272）序列及图谱，有这两个质粒信息的小伙伴麻烦告知一下啦，十分感谢 ...
WebJun 9, 2024 · 求BacmidBMON14272图谱或序列;求BacmidBMON14272图谱或序列;你可以参考一下这个公司给的说明书，他们不让我贴连接，我把pdf的头给你贴上。你自己找来看 …
WebAug 13, 2014 · 1 Answer. It seems that no good map of this plasmid is around. Life technology uses it in some of its bacterial strains, the quote: E. coli also contain the helper plasmid, pMON7124 (13.2 kb), which encodes the transposase and confers resistance to tetracycline. The helper plasmid provides the Tn7 transposition function in trans. selling salvage title cars overseasWebApr 20, 2024 · 普通的 Java 应用或者 Java 库使用 BOM 的时候需要先添加 BOM 依赖，然后使用其它的库。. 例如：. // 引入 BOM. implementation platform (“org.example:third-part … selling saffron onlineWebJun 9, 2024 · 求BacmidBMON14272图谱或序列;求BacmidBMON14272图谱或序列;你可以参考一下这个公司给的说明书，他们不让我贴连接，我把pdf的头给你贴上。你自己找来看看吧; 48小时，有问必答 ... 求Bacmid BMON14272 图谱或序列 selling saffron priceWebNov 10, 2013 · 杆状病毒表达系统helper质粒与Bacmid质粒序列及图谱. 如题，求助杆状病毒表达系统helper质粒（pMON7124）与Bacmid质粒（bMON14272）序列及图谱，有这 … selling salvage cars durham ncWebOct 21, 2024 · get LB-Agar plate from cold room and leave on bench to get to roomtemp. get desired Plasmid from -20°C and thaw on ice. get DH10Bac vial from -80 and thaw on ice for 10min. add 5-50ng of plasmid to 1x 50 µL vial of DH10Bac competent cells. mix by stirring gently with the pipette tip, do not vortex or mix by pipetting up and down. selling sample only pastriesWebNov 28, 2004 · This vector was transformed into DH10BAC (Invitrogen) cells harboring the bacmid bMON14272 and the helper plasmid pMON7124 17,18 yielding colonies resistant to kanamycin, tetracyclin and zeocin. A ... selling sample script for promoting a productWebDH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between … selling samosas from home