WebIts molecular mass is 27 kD. [7] Therefore, fusing the GFP gene to the gene of a protein of interest can significantly increase the protein's size and molecular mass, and can impair the protein's natural function or change … WebIts excitation peak is 513 nm and its emission peak is 527 nm. [2] Like the parent GFP, YFP is a useful tool in cell and molecular biology because the excitation and emission peaks of YFP are distinguishable from GFP …
Introduction and Photo Spectral Characteristics - Leica …
WebApr 9, 2012 · This ECFP has a bimodal excitation and emission spectrum at 433/445 nm and 475/503 nm. Brightness is only about 40 % of that of EGFP. A prominent ECFP variant is Cerulean, which has a higher extinction coefficient and quantum yield. It is 1.5 times brighter than ECFP and is used as a FRET partner with YFP. WebYeast cells were transfected with genes for yellow fluorescent protein (YFP) or cyan fluorescent protein ( CFP )–tagged PMPs or CFP fused to the PTS ( CFP-PTS) under control of a GAL1 promoter. Transfected cells were grown in YP media containing 2% galactose for 2 h at 30°C. mark camilleri twitter
Fluorescent protein tags Proteintech Group - ptglab
WebLength/ molecular weight (MW) 297 amino acids, 33.6 kDa. 182 amino acids, 19.4 kDa. 182 amino acids, 19.4 kDa. Research tools offered by ChromoTek. Immunoprecipitation: Halo-Trap Western blotting: mouse anti-HaloTag antibody [28A8] Immunoprecipitation: SNAP/CLIP-tag-Trap Western blotting: rat anti-SNAP/CLIP-tag ® antibody [6F9] WebMolecular Weight Cofactor Monomer Aequorea victoria 26.9 kDa FPbase ID: WCSN6 Attributes Ex λ Em λ EC(M-1cm-1) QY Brightness pKa Maturation (min) Lifetime (ns) 515 527 104,000 0.64 66.56 5.5 17.6 2.7 Edit States/AttributesEdit States/Attributes mVenus OSER Measurements Photostability WebEYFP is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer with high acid sensitivity. Oligomerization Organism Molecular Weight Cofactor Weak dimer Aequorea victoria 27.0 kDa FPbase ID: 8DNLG Attributes Ex λ Em λ mark campbell 2023 schedule