Click-it edu cell proliferation kit alexa 594

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August undefined, 2024

WebThe Click-iT EdU Alexa Fluor 594 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5 … WebMar 9, 2015 · Alexa Fluor® 594 azide: 590/615; Alexa Fluor® 647 azide: 650/670; Hoechst 33342: 350/461, bound to DNA. ... including dyes for cell cycle analysis. The EdU assay kit can also be multiplexed with ... If you are currently using a BrdU-based assay for cell proliferation, a similar concentration to BrdU is a good starting concentration for EdU.

Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa …

WebParaffin sections (5 µm thick) were processed to detect Edu-positive cells, and cell proliferation was analysed using the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Thermo Fisher Scientific). For analysis of cellular apoptosis, TUNEL staining was performed using the In situ Apoptosis Detection Kit (Takara Bio) according to the ... Web(C) In contrast, Erk2-GFP A375 cells processed using the reagents and fixation/detection protocol provided in the Click-iT Plus EdU Alexa Fluor 594 Proliferation Assay Kit retained their GFP signal (green), and the EdU-based detection of proliferation was very bright (red, 8 msec exposure). Both cell samples were treated with Hoechst 33342 ... mercury 1000 tube tester chart

Click-iT EdU Imaging Kit with Alexa Fluor 488, 594, and 647 …

Webcells were detected with the Click-iT® EdU Alexa Fluor® 594 Imaging Kit (Cat. no. C10339). Nuclei were stained with the blue-fluorescent counterstain Hoechst 33342 (Cat. no. H1399). EdU 4 ... BrdU protocol, cells are first click labeled for the detection of EdU followed by an antibody WebClick-iT® EdU cell proliferation assays eliminate DNA denaturation steps required by BrdU assays, steps that can damage sample morphology and integrity. This trial-size kit provides sufficient EdU to ... EdU Alexa Fluor® 594 Imaging Kit (red-fluorescence, C10339). Mitochondria are pseudocolored pink. WebThe second step of EdU experiment was EdU labeling: after overnight incubation, we used BeyoClick TM EdU-594 cell proliferation detection kit (C0078S, Beyotime) for EdU experiment. According to the instruction of the kit, we diluted the EdU stock (10 mM) with culture medium (10% fetal bovine serum) at a ratio of 1:500 to obtain the 2x EdU ... mercury 1000 tube tester diagram

Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa …

IJMS Free Full-Text EN1 Regulates Cell Growth and Proliferation …

Webmethod. In contrast, the Click-iT™ Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. Figure 1 (below) shows Click-iT™ Plus EdU Alexa Fluor™ 488 multiplexed with R-PE, Figure 2 (page 3) demonstrates Click-iT™ Plus EdU Alexa Fluor™ 350 Webmethod. In contrast, the Click-iT® Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. Figure 2 below demonstrates Click-iT® Plus EdU multiplexed with Hu CD3 PE and Figure 3 (page 3) shows Click-iT® Plus EdU multiplexed with FxCycle™ violet and … how old is hong kongWebEdU staining was conducted using the BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 594 (Beyotime, Cat. No: C00788L). Cells were washed with PBS. Fresh DMEM was added, and then, 10 μM EdU was added into the medium. The cells were incubated for 2 hours at 37°C/5% CO 2. After the incubation, the cells were washed with PBS to remove ... how old is hook aew

"WebThe Click-&-Go ® EdU 594 Flow Cytometry Assay Kit is a superior alternative to the traditional cell proliferation assays that use radioactive nucleosides, i.e., 3H-thymidine or antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog of thymidine with a terminal alkyne group … " - Click-it edu cell proliferation kit alexa 594

Click-it edu cell proliferation kit alexa 594

Click-iT™ Plus EdU Cell Proliferation Kit for Imaging ...

WebClick-&-Go EdU Cell Proliferation Assays optimized for measuring cell’s ability to proliferate based on direct DNA synthesis monitoring. Products. ... The Click-&-Go EdU 594 Flow Cytometry Assay Kit is a simplified, robust assay for analyzing DNA replication in proliferating cells. Ideally suited for the 532- or 561-nm laser of the flow ... WebApr 12, 2024 · On day 5, 10 μM EdU was added to the cells and incubated at 37°C for 2 hours. EdU incorporation was measured using a Click-it Plus EdU Alexa Fluor 594 Imaging Kit (Invitrogen) in accordance with the manufacturer’s instructions. Images were captured on a Zeiss Observer microscope.

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WebInvitrogen™ Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye. Designed to label and detect incorporated EdU and perform cell cycle analysis on … WebArticle Title: Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Journal: Cell Stem Cell. ... Click It Edu Alexa Fluor 594 Image Kit, supplied by …

WebSuperior Proliferation Methodology The Click-iT EdU Alexa Fluor 594 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5 … WebEdU Cell Proliferation Assay EdU-488 Catalog No. 17-10525 EdU-555 Catalog No. 17-10526 EdU-594 Catalog No. 17-10527 EdU-647 Catalog No. 17-10528 100 reactions FOR RESEARCH USE ONLY Not for use in diagnostic procedures. USA & Canada Phone: +1(800) 645-5476 In Europe, please contact Customer Service: France: 0825.045.645; …

WebPrepare stock solutions. Add 2 mL DMSO (Component C) or an aqueous solution to Component A to make a 10 mM EdU stock solution. Store at –20°C. Make 1X Click-iT® EdU reaction buffer by adding 36 mL of deionized water to Component D. Store at 2–8° C. Make Alexa Fluor® azide by adding 70 μL DMSO (Component C) to Component B, then … WebContents & Storage. Contains EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor™ dye-picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ EdU reaction buffer, Copper Protectant, Click-iT™ EdU buffer additive, …

WebDec 30, 2024 · Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594: Thermo Fisher Scientific: Cat#C10339: Live/Dead Blue kit: Thermo Fisher Scientific: Cat#L34961: Software and algorithms: FlowJo: BD Life Sciences: n/a: Other: Vicell Cell counter: Beckman Coulter: n/a: Dissection tool: 14.5 mm scissors: Fine Science Tools: …

WebMar 9, 2015 · In contrast, the EdU cell proliferation kit is compatible with cell cycle dyes (Figure 2). The EdU assay can also be multiplexed with antibodies against surface and intracellular markers. However, some reagents or antibody conjugates may not be compatible with the Click-iT® EdU detection reaction and may need some additional … mercury 1000 tube testerWebClick-iT Plus EdU Alexa Fluor imaging kits have been optimized for fluorescence microscopy applications and are a superior alternative to traditional proliferation assays. … mercury 1000 induction cookerWebCellTrace CFSE Cell Proliferation Kit ... ERK2 A375 GFP expressing cells labeled with Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (pink), Proliferating cells have pink nuclei. Non-proliferating cells have blue nuclei. ... Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647; Excitation/Emission (in nm) 350/461: 495/519: 555/565: 590/615: 650/670 ... how old is honkaiWebClick-&-Go ® EdU 594 Cell Proliferation Assay includes blue fluorescent Hoechst 33342 dye for performing cell cycle analysis on samples from adherent cells. Sufficient amount … mercury 1000w bt 56 bt56WebJul 23, 2024 · To analyze the ATDC5 proliferation after transfection, EdU staining was conducted using the BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 594 (Beyotime Biotechnology, Jiangsu, China, Cat. No. C00788S) according to the manufacturer’s instructions. mercury 1000 outboard partsWebClick-&-Go® Plus EdU Cell Proliferation Assays Click-&-Go® Plus EdU Flow Cytometry Kits Click-&-Go® EdU Flow Cytometry Kits. ... 594 Laser line Tx Red Common filter set. Catalog #: 1328 -+ $ 249.00. Add to cart. Click ... how old is hooniWebMar 9, 2015 · In contrast, the EdU cell proliferation kit is compatible with cell cycle dyes (Figure 2, page 3). The EdU assay can also be multiplexed with antibodies against surface and intracellular markers. However, some reagents or antibody conjugates may not be compatible with the Click-iT® EdU detection reaction and may need some additional mercury 100 gram price