WebJan 5, 2024 · The CTB assay was performed in hepatocytes of at least 3 different human donors each with 3–4 technical replicates. 2.4. CMFDA assay. For a detailed standard operating procedure (SOP) see Supplement 2. In brief, the day after seeding, cells were incubated for 1 h with the corresponding compound of interest followed by 20 min … Web报告基因是一类使表达产物易于被检测的基因,其作用原理是将报告基因的编码序列及其表达调控序列与目的基因融合形成嵌合基因,在调控序列的作用下进行表达,通过对报告基因表达产物的检测来评价目的基因的表达状况。 常见的报告基因包括萤光素酶(Luciferase)、氯霉素乙酰转移酶(CAT ...
Materials Free Full-Text Xeno-Free In Vitro Cultivation and ...
WebJul 31, 2024 · CTB assay was performed according to the information provided by the manufacturer. Briefly, a 10% CTB solution was prepared with α-MEM (1:10 v/v) and 500 µL were added to each well after carefully aspirating the old medium. Additionally, wells without cells were filled with the 10% CTB solution and used as a blank. The fluorescence was ... WebThe CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a … florida hotels with kitchenette
Frontiers Commentary: Development of a Comparative European ...
WebNov 2, 2015 · These two methods are independent of mitochondrial activity. The results are consistent with those acquired via CTB assays (please see new Figure 1B), indicating that the CTB assay is a reliable method to examine acid-induced cell death under our experimental conditions. So far, we have no evidence supporting that mtASIC1a directly … WebNov 5, 2024 · Alternative assays using fluorescent or colorimetric dyes such as, Cell Titer Blue (CTB), Propidium Iodide (PI), Calcein AM, 2′,7′-dichlorofluorescin diacetate (DCFDA), and Annexin V labeled ... WebThis CTB will serve to provide all information and instructions associated with implementation and performance of the uncapped workflow for testing with the Aptima SARS-CoV-2 assay. Information contained in this CTB will be incorporated into future revisions of the appropriate Aptima SARS-CoV-2 assay associated package inserts. … great wall palm coast florida