Flag-tag mouse monoclonal antibody

Author: mwye

August undefined, 2024

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. WebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging …

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WebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application WebProduct Specific Information. This antibody detects Flag tag from various samples. similar epitope as the FLAG tag from Sigma. MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications. The MA1 … The membrane was probed with a DyLight 800-conjugated FLAG Epitope Tag … Published figure using DYKDDDDK Tag monoclonal antibody (Product # MA1 … biological aspects of psychology

FLAG M2 mouse monoclonal antibody (DYKDDDDK tag).

WebTHE™ DYKDDDDK Tag Antibody, mAb, Mouse THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins. Product Details Citations Documents Overview Applications Properties Examples Background For laboratory research use only. WebDYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic … WebHA-tag Rabbit Monoclonal Antibody staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. ... HA Tag Mouse Monoclonal Antibody. AG8050. 50μl. Flag Tag Rabbit … biological assessment usfws

DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488

WebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... Monoclonal ANTI-FLAG? M2 … WebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. biological assay methodshttp://www.abpbiosciences.com/product/flag-tag/ daily mail schools rugby cup

"WebFLAG Tag Rabbit Polyclonal Antibody: 100μl: AF5051: Flag Tag Mouse Monoclonal Antibody: 50μl: AF519: Flag抗体(小鼠单抗) >40次: AF0033: Myc Tag Mouse Monoclonal Antibody: 100μl: AF5054: Myc Tag Mouse Monoclonal Antibody: 50μl: AF6513: c-Myc Rabbit Polyclonal Antibody: 50μl: AM926: " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

DYKDDDDK tag antibody (66008-4-Ig) Proteintech - ptglab

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived …

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WebConfocal image showing positive staining for FLAG® on HEK-293T cells transfected with DDDDK-tagged PFKFB3 expression vector. Mouse … WebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano …

WebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. … Web3 hours ago · Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, Burlington, MA, USA, 1:10.000 dilution) for 1 h at RT. ... or an established mouse IgG2 monoclonal antibody recognizing PSD95. In these indirect setups, after washing (3 × 5 min in PBS each), the anti-PSD95 r-hcAb or …

WebAntibodies that detect DYKDDDDK Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and …

WebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species …

WebWestern blot was performed using HRP-conjugated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), HA-H3-FLAG, 40 µg (Lane 3), HA-H3-FLAG, 20 µg (Lane 4), HA … biological aspects of personalityWebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … daily mail- september 9th 2013WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and … daily mail scottish newsWebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ... dailymail shana eversonWebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB … biological asset mfrs 141WebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer. daily mail scotland newshttp://www.abpbiosciences.com/product/flag-tag/ daily mail scottish edition