Pheochromocytoma pc12 cells

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August undefined, 2024

WebApr 13, 2024 · Pheochromocytoma 12 (PC12) cells derived from rat adrenal glands were purchased from ATCC (Manassas, VA, USA) and used for the experiments. PC12 cells were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C in a 5% CO 2 incubator. The medium … WebPheochromocytoma PC12 cells were obtained from the Bioresources Collection and Research Center (BCRC-60048; Hsinchu, Taiwan) and maintained in the RPMI-1640 medium supplemented with 10% heat-inactivated horse serum and 5% FBS. All the protocols were approved by the National Cheng-Kung University Investigation Committee.

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WebJan 31, 2024 · The PC 12 cells remained viable and physiologically healthy, as confirmed by a panel of biological assays; however, exposure to THz radiation for 10 min at 25.2 ± 0.4 … WebApr 11, 2024 · Cocaine. and amphetamine-regulated transcript peptide (CARTp) is an anorexigenic neuropeptide whose receptor is undisclosed. Previously, we reported the specific binding of CART(61–102) to pheochromocytoma PC12 cells, where CART(61–102) affinity and the number of binding sites per cell corresponded to ligand-receptor binding. greiner investmentsllc com filetypepdf

Regulation of the differentiation of PC12 pheochromocytoma cells …

WebJan 1, 1997 · We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP+), paraquat, and 1-methyl-4-(4'-nitrophenyl) pyridinium … WebMar 18, 2024 · This study aimed to determine the effects of reperfusion in an in vitro ischaemic stroke model—oxygen and glucose deprivation (OGD) (0.3% O2)—with rat pheochromocytoma (PC12) cells and cortical neurons. In PC12 cells, OGD resulted in a time-dependent increase in cytotoxicity and apoptosis, and reduction in MTT activity from … WebJun 14, 2013 · Tunneling nanotubes (TNTs) are thin cell-to-cell connected membranous tubes first described in rat pheochromocytoma (PC12) cells. 1 Subsequent studies demonstrated that TNTs are present in a... greiner insurance agency

Transfection of PC12 cells with the human GAP-43 gene: effects …

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WebJun 14, 2013 · Tunneling nanotubes (TNTs) are thin cell-to-cell connected membranous tubes first described in rat pheochromocytoma (PC12) cells. 1 Subsequent studies … WebMar 18, 2024 · This study aimed to determine the effects of reperfusion in an in vitro ischaemic stroke model—oxygen and glucose deprivation (OGD) (0.3% O 2 )—with rat … fiche projet bts communicationWebAug 22, 2013 · The SF16 hydrogel scaffold was characterized by analysis with atomic force microscopy (AFM) and scanning electron microscopy (SEM), and was assessed in terms of its ability to maintain the viability, morphology and proliferation of PC12 rat pheochromocytoma cells in vitro. fiche projet cycle 3

"WebThe pheochromocytoma (PC12) cell line is commonly used in in vitro studies to examine neuronal differentiation and neurotoxicity implicated in neurodegenerative disease [ 1, 2 ]. PC12 cells are noradrenergic clonal cells originating from Rattus norvegicus transplantable pheochromocytoma [ 1 ]. " - Pheochromocytoma pc12 cells

Pheochromocytoma pc12 cells

WebPC12 cell line is well documented and widely applied as many kinds of models in neurobiological and neurochemical studies. Yet a thorough proteomic analysis has not … WebFeb 21, 2024 · TNTs were first described in rat-cultured pheochromocytoma PC12 cells , and subsequent studies have shown that they connect a wide variety of cell types. These studies provide more evidence that TNTs are involved in mitochondrial transport between cells, the repair of cell damage, the activation of enhanced immune responses, and cell …

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WebJan 6, 2015 · Vesicular exocytosis is ubiquitous, but it is difficult to detect within the cells’ communication mechanism. For this purpose, a 2 µm ultramicrodic carbon fiber electrode was fabricated in this work based on electrodeposition with over-oxidized polypyrrole nanoparticle (PPyox-CFE), which was applied successfully for real-time monitoring of … WebThe catecholamine-secreting PC12 cell line derived from a rat pheochromoytoma originating from chromaffin cells of the adrenal medulla has long been considered a suitable model system for studying neurosecretion and neuronal differentiation.

WebThe PC12 clone, developed from a pheochromocytoma tumor of the rat adrenal medulla, has become a premiere model for the study of neuronal differentiation. WebFigure Legend Snippet: PC12 cells were serum-deprived for 16 h and subsequently treated with NGF (50 ng/ml) for 72 h. A. NGF induced progressive neurite outgrowth in PC12 cells. Percentage of PC12 cells bearing neurite twice the body length (differentiated) was quantified after 24, 48 and 72 h of NGF treatment.

WebDec 28, 2024 · We have identified FA as a molecule that inhibits apoptosis induced by hydrogen peroxide (H 2 O 2) or actinomycin D (ActD) in rat pheochromocytoma, PC12 cell. We also found that FA reduces H 2 O 2 -induced reactive oxygen species (ROS) production in PC12 cell, thereby acting as an anti-oxidant. Then, we analyzed FA-mediated signaling … WebApr 14, 2024 · The PC12 pheochromocytoma cell line of neoplastic chromaffin cells was established from a rat adrenal tumour in 1976 1. Treatment of these cells with nerve …

WebMar 31, 1995 · Nerve growth factor (NGF) induces both differentiation and survival of neurons by binding to the Trk receptor protein tyrosine kinase. Although Ras is required for differentiation, it was not required for NGF-mediated survival of rat pheochromocytoma PC-12 cells in serum-free medium.

WebDec 16, 2011 · (PDF) Phospholipase A2 and Signaling Pathways in Pheochromocytoma PC12 Cells Phospholipase A2 and Signaling Pathways in Pheochromocytoma PC12 Cells Authors: Alexey Osipov Yuri N Utkin Russian... fiche projet certification ifs 7WebPC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core … greiner landscaping ormond beachWebNational Center for Biotechnology Information fiche promis cnrsWebThe rat pheochromocytoma PC12 cell line can easily be manipulated to study neuronal differentiation and apoptosis. When cells are cultured with serum, addition of PACAP inhibits cell proliferation and induces neurite outgrowth. When cells are cultured in serum-free medium, PACAP promotes PC12 survival and neuritogenesis. ... fiche promontofixationWebThe PC12 clone, developed from a pheochromocytoma tumor of the rat adrenal medulla, has become a premiere model for the study of neuronal differentiation. fiche projet exemple wordWebJan 1, 1987 · This chapter presents several details regarding its culture and experimental exploitation—in particular, methods for the maintenance and nerve growth factor (NGF) … greiner lithium heparin tubeWebOct 8, 2024 · Rat Pheochromocytoma PC12 Cells in Culture 1 Introduction. Two types of vesicles, synaptic vesicles (SVs) and dense-core vesicles (DCVs), have been found in … greiner law firm